EXOSC7 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1G8]

CAT#: TA504239BM

EXOSC7 mouse monoclonal antibody, clone OTI1G8 (formerly 1G8), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EXOSC7 (Cat# RC201419, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EXOSC7(Cat# TA504239). Positive lysates LY402399 (100ug) and LC402399 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EXOSC7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-EXOSC7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504239)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-EXOSC7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504239)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-EXOSC7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504239)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EXOSC7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504239)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EXOSC7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504239)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EXOSC7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504239)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EXOSC7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504239)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-EXOSC7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504239)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-EXOSC7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504239)

• Anti-EXOSC7 mouse monoclonal antibody (TA504239) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EXOSC7 (RC201419).

• HEK293T cells transfected with either RC201419 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EXOSC7 antibody (TA504239), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-EXOSC7 antibody (TA504239), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1G8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EXOSC7(NP_055819) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.6 kDa
• Gene Name: exosome component 7
• Database Link:
  NP_055819
  Entrez Gene 66446 MouseEntrez Gene 316098 RatEntrez Gene 476654 DogEntrez Gene 714624 MonkeyEntrez Gene 23016 Human
  UniProt ID Q15024
• Synonyms: EAP1; hRrp42p; p8; RRP42; Rrp42p

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation