FBXO21 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1E1]

CAT#: TA503940AM

FBXO21 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO21 (RC223095, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO21.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503940)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503940)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503940)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503940)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503940)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FBXO21 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503940)

• Anti-FBXO21 mouse monoclonal antibody (TA503940) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FBXO21 (RC223095).

• HEK293T cells transfected with either RC223095 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FBXO21 antibody (TA503940), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-FBXO21 antibody (TA503940), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-FBXO21 antibody (TA503940), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1E1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FBXO21(NP_296373) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.1 kDa
• Gene Name: F-box protein 21
• Database Link:
  NP_296373
  Entrez Gene 231670 MouseEntrez Gene 360818 RatEntrez Gene 23014 Human
  UniProt ID O94952
• Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]
• Synonyms: FBX21