ERp72 (PDIA4) Mouse Monoclonal Antibody [Clone ID: OTI2F1]

CAT#: TA503883

PDIA4 (ERp72) mouse monoclonal antibody, clone OTI2F1 (formerly 2F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA4 (RC204041, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA4. Positive lysates LY401531 (100ug) and LC401531 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDIA4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PDIA4-Knockout 293T cells (KO, Cat# LC841833) were separated by SDS-PAGE and immunoblotted with anti-PDIA4 monoclonal antibody TA503883, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

Specifications

Product Data

• Clone Name: OTI2F1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDIA4(NP_004902) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.52 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.8 kDa
• Gene Name: protein disulfide isomerase family A member 4
• Database Link:
  NP_004902
  Entrez Gene 12304 MouseEntrez Gene 116598 RatEntrez Gene 9601 Human
  UniProt ID P13667
• Synonyms: ERp-72; ERP70; ERP72

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vibrio cholerae infection