Lipoamide Dehydrogenase (DLD) Mouse Monoclonal Antibody [Clone ID: OTI8A10]

CAT#: TA503388

DLD (Lipoamide Dehydrogenase) mouse monoclonal antibody, clone OTI8A10 (formerly 8A10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (RC200639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD. Positive lysates LY400041 (100ug) and LC400041 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DLD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and DLD-Knockout HeLa cells (KO, Cat# LC832718) were separated by SDS-PAGE and immunoblotted with anti-DLD monoclonal antibody TA503388 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  HEK293T cells transfected with either RC200639 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DLD antibody (TA503388), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI8A10
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DLD(NP_000099) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.89 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: dihydrolipoamide dehydrogenase
• Database Link:
  NP_000099
  Entrez Gene 13382 MouseEntrez Gene 298942 RatEntrez Gene 403978 DogEntrez Gene 700494 MonkeyEntrez Gene 1738 Human
  UniProt ID P09622
• Background: This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq, Jul 2008]
• Synonyms: DLDD; DLDH; E3; GCSL; LAD; PHE3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glycine, serine and threonine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine degradation