PTP1B (PTPN1) Mouse Monoclonal Antibody [Clone ID: OTI1D10]

CAT#: TA503310S

PTPN1 (PTP1B) mouse monoclonal antibody, clone OTI1D10 (formerly 1D10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PTPN1 (Cat# RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1(Cat# TA503310). Positive lysates LY419087 (100ug) and LC419087 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN1 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and PTPN1-Knockout Hela cells (KO, Cat# LC810193) were separated by SDS-PAGE and immunoblotted with anti-PTPN1 monoclonal antibody TA503310. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503310)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503310)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503310)

• Anti-PTPN1 mouse monoclonal antibody (TA503310) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1 (RC204902).

• HEK293T cells transfected with either RC204902 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PTPN1 antibody (TA503310), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1D10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Monkey, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PTPN1(NP_002818) produced in HEK293 cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.8 kDa
• Gene Name: protein tyrosine phosphatase non-receptor type 1
• Database Link:
  NP_002818
  Entrez Gene 24697 RatEntrez Gene 477263 DogEntrez Gene 702016 MonkeyEntrez Gene 5770 Human
  UniProt ID P18031
• Background: The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq]
• Synonyms: PTP1B

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Adherens junction, Insulin signaling pathway