PNMT Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1B11]

CAT#: TA502854AM

PNMT mouse monoclonal antibody, clone OTI1B11 (formerly 1B11), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (RC206586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNMT. Positive lysates LY400946 (100ug) and LC400946 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502854)

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  Anti-PNMT mouse monoclonal antibody (TA502854) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT (RC206586 ).

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  Immunofluorescent staining of HeLa cells using anti-PNMT mouse monoclonal antibody (TA502854).

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  HEK293T cells transfected with either RC206586 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PNMT antibody (TA502854), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-PNMT antibody (TA502854), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-PNMT antibody (TA502854), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1B11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PNMT (NP_002677) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.7 kDa
• Gene Name: phenylethanolamine N-methyltransferase
• Database Link:
  NP_002677
  Entrez Gene 18948 MouseEntrez Gene 5409 Human
  UniProt ID P11086
• Background: The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq]
• Synonyms: PENT; PNMTase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tyrosine metabolism