SULT1C2 Mouse Monoclonal Antibody [Clone ID: OTI5A4]

CAT#: TA502665S

SULT1C2 mouse monoclonal antibody, clone OTI5A4 (formerly 5A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT1C2 (RC202775, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT1C2. Positive lysates LY420736 (100ug) and LC420736 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-SULT1C2 monoclonal antibody (1:200).

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  Figure from citation: Western Blot of SULT1C2 protein level by using anti-SULT1C2 antibody in human LS180 cells. Dilution: 1:2000 View Citation

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SULT1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502665)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SULT1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502665)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SULT1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502665)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SULT1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502665)

• HEK293T cells transfected with either RC202775 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SULT1C2 antibody (TA502665), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-SULT1C2 antibody (TA502665), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI5A4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SULT1C2 (NP_001047) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.7 kDa
• Gene Name: sulfotransferase family 1C member 2
• Database Link:
  NP_001047
  Entrez Gene 6819 Human
  UniProt ID O00338
• Background: Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that belongs to the SULT1 subfamily, responsible for transferring a sulfo moiety from PAPS to phenol-containing compounds. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
• Synonyms: humSULTC2; ST1C1; ST1C2; SULT1C1