Beta TRCP (BTRC) Mouse Monoclonal Antibody [Clone ID: OTI3D5]

CAT#: TA502464

BTRC (Beta TRCP) mouse monoclonal antibody, clone OTI3D5 (formerly 3D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTRC (RC207025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTRC.

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  Anti-BTRC mouse monoclonal antibody (TA502464) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTRC (RC207025).

Specifications

Product Data

• Clone Name: OTI3D5
• Applications: IF, WB
• Recommended Dilution: WB 1:500, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human fragment corresponding to amino acids 52-354 of human BTRC (NP_378663) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.75 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.7 kDa
• Gene Name: beta-transducin repeat containing E3 ubiquitin protein ligase
• Database Link:
  NP_378663
  Entrez Gene 12234 MouseEntrez Gene 361765 RatEntrez Gene 8945 Human
  UniProt ID Q9Y297
• Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway. [provided by RefSeq]
• Synonyms: BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Hedgehog signaling pathway, Oocyte meiosis, Ubiquitin mediated proteolysis, Wnt signaling pathway