EIF4EL3 (EIF4E2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5D11]

CAT#: TA502430AM

EIF4E2 mouse monoclonal antibody, clone OTI5D11 (formerly 5D11), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E2 (RC201391, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E2. Positive lysates LY401518 (100ug) and LC401518 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF4E2 monoclonal antibody.

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  Anti-EIF4E2 mouse monoclonal antibody (TA502430) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF4E2 (RC201391).

Specifications

Product Data

• Clone Name: OTI5D11
• Applications: IF, WB
• Recommended Dilution: WB 1:1000~2000, IF 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EIF4E2 (NP_004837) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.2 kDa
• Gene Name: eukaryotic translation initiation factor 4E family member 2
• Database Link:
  NP_004837
  Entrez Gene 26987 MouseEntrez Gene 9470 Human
  UniProt ID O60573
• Synonyms: 4E-LP; 4EHP; EIF4EL3; IF4e

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway