PDE2A Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3A4]

CAT#: TA502389AM

PDE2A mouse monoclonal antibody, clone OTI3A4 (formerly 3A4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A. Positive lysates LY419215 (100ug) and LC419215 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502389)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDE2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502389)

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  Anti-PDE2A mouse monoclonal antibody (TA502389) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE2A (RC207219).

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  HEK293T cells transfected with either RC207219 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE2A antibody (TA502389), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-PDE2A antibody (TA502389), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI3A4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDE2A (NP_002590) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 105.5 kDa
• Gene Name: phosphodiesterase 2A
• Database Link:
  NP_002590
  Entrez Gene 81743 RatEntrez Gene 207728 MouseEntrez Gene 5138 Human
  UniProt ID O00408
• Synonyms: CGS-PDE; cGSPDE; PDE2A1; PED2A4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism