Gephyrin (GPHN) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3B6]

CAT#: TA502217AM

GPHN (Gephyrin) mouse monoclonal antibody, clone OTI3B6 (formerly 3B6), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN. Positive lysates LY402806 (100ug) and LC402806 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502217)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502217)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502217)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502217)

• Anti-GPHN mouse monoclonal antibody (TA502217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GPHN (RC205986).

• HEK293T cells transfected with either RC205986 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GPHN antibody (TA502217), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-GPHN antibody (TA502217), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI3B6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GPHN (NP_065857) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83.3 kDa
• Gene Name: gephyrin
• Database Link:
  NP_065857
  Entrez Gene 64845 RatEntrez Gene 268566 MouseEntrez Gene 709677 MonkeyEntrez Gene 10243 Human
  UniProt ID Q9NQX3
• Background: This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq]
• Synonyms: GEPH; GPH; GPHRYN; HKPX1; MOCODC

Reference Data

• Protein Families: Druggable Genome