MYD88 Mouse Monoclonal Antibody [Clone ID: OTI1B8]

CAT#: TA502118S

MYD88 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYD88 (RC202253, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYD88 (1:500).

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  Western blot analysis of extracts (35ug) from 3 cell lines lysates by using anti-MYD88 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MYD88 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502118)

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  Anti-MYD88 mouse monoclonal antibody (TA502118) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MYD88 (RC202253) (1:100).

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  HEK293T cells transfected with either RC202253 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYD88 antibody (TA502118), and then analyzed by flow cytometry (1:100).

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Specifications

Product Data

• Clone Name: OTI1B8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Rat, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MYD88 (NP_002459) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.4 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.1 kDa
• Gene Name: MYD88 innate immune signal transduction adaptor
• Database Link:
  NP_002459
  Entrez Gene 301059 RatEntrez Gene 696494 MonkeyEntrez Gene 4615 Human
  UniProt ID Q99836
• Background: This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: MYD88D

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Toll-like receptor signaling pathway