SAMHD1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D7]

CAT#: TA501954BM

SAMHD1 mouse monoclonal antibody, clone OTI2D7 (formerly 2D7), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1. Positive lysates LY414526 (100ug) and LC414526 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-SAMHD1 monoclonal antibody at 1:200 dilution.

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  Figure from citation: Western Blot of SAMHD1 protein level by using anti-SAMHD1 antibody in lysates from parental U937 or U937 expressing human or mouse SAMHD1. Dilution: 1:1000 View Citation

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  Anti-SAMHD1 mouse monoclonal antibody (TA501954) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1 (RC206013).

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  Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody (TA501954), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-SAMHD1 antibody (TA501954), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2D7
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SAMHD1 (NP_056289) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72 kDa
• Gene Name: SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1
• Database Link:
  NP_056289
  Entrez Gene 25939 Human
  UniProt ID Q9Y3Z3
• Background: This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: CHBL2; DCIP; HDDC1; MOP-5; SBBI88