IVD Mouse Monoclonal Antibody [Clone ID: OTI1B10]

CAT#: TA501742S

Anti-IVD mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IVD (Cat# RC201077, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IVD(Cat# TA501742). Positive lysates LY432239 (100ug) and LC432239 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IVD monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IVD mouse monoclonal antibody (TA501742) at 1:150 dilution.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IVD mouse monoclonal antibody (TA501742) at 1:150 dilution.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-IVD mouse monoclonal antibody (TA501742) at 1:150 dilution.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IVD mouse monoclonal antibody (TA501742) at 1:150 dilution.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IVD mouse monoclonal antibody (TA501742) at 1:150 dilution.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-IVD mouse monoclonal antibody (TA501742) at 1:150 dilution.

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-IVD mouse monoclonal antibody (TA501742) at 1:150 dilution.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IVD mouse monoclonal antibody (TA501742) at 1:150 dilution.

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-IVD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501742, Dilution 1:50)

• Anti-IVD mouse monoclonal antibody (TA501742) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IVD (RC201077).

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Specifications

Product Data

• Clone Name: OTI1B10
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IVD (NP_002612) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.5 kDa
• Gene Name: isovaleryl-CoA dehydrogenase
• Database Link:
  NP_002216
  Entrez Gene 24513 RatEntrez Gene 56357 MouseEntrez Gene 100856316 DogEntrez Gene 702867 MonkeyEntrez Gene 3712 Human
  UniProt ID P26440
• Background: Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: ACAD2; IVDH

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation