MCL1 Mouse Monoclonal Antibody [Clone ID: OTI9D7]

CAT#: TA500985

Anti-MCL1 mouse monoclonal antibody, clone OTI9D7 (formerly 9D7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1. Positive lysates LY411855 (100ug) and LC411855 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-MCL1 monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500985, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500985, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500985, Dilution 1:50)

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  Anti-MCL1 mouse monoclonal antibody (TA500985) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1 (RC200521).

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Specifications

Product Data

• Clone Name: OTI9D7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:50, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.81 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.3 kDa
• Gene Name: MCL1 apoptosis regulator, BCL2 family member
• Database Link:
  NP_068779
  Entrez Gene 4170 Human
  UniProt ID Q07820
• Background: The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
• Synonyms: bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM

Reference Data

• Protein Families: Druggable Genome, Transmembrane