RPA34 (RPA2) Mouse Monoclonal Antibody [Clone ID: OTI7F4]

CAT#: TA500812S

Anti-RPA2 mouse monoclonal antibody, clone OTI7F4 (formerly 7F4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2. Positive lysates LY401031 (100ug) and LC401031 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500812, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500812, Dilution 1:50)

Specifications

Product Data

• Clone Name: OTI7F4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.67 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.2 kDa
• Gene Name: replication protein A2
• Database Link:
  NP_002937
  Entrez Gene 19891 MouseEntrez Gene 59102 RatEntrez Gene 6118 Human
  UniProt ID P15927
• Background: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions
• Synonyms: REPA2; RP-A p32; RP-A p34; RPA32

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair