PSMD10 Mouse Monoclonal Antibody [Clone ID: OTI3F6]

CAT#: TA500781

Anti-PSMD10 mouse monoclonal antibody, clone OTI3F6 (formerly 3F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PSMD10 (Cat# RC202025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD10(Cat# TA500781). Positive lysates LY419094 (100ug) and LC419094 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD10 monoclonal antibody.

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  Flow cytometric analysis of Hela cells, using anti-PSMD10 antibody (TA500781), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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  Flow cytometric analysis of Jurkat cells, using anti-PSMD10 antibody (TA500781), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

Specifications

Product Data

• Clone Name: OTI3F6
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PSMD10 (NP_002805) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.4 kDa
• Gene Name: proteasome 26S subunit, non-ATPase 10
• Database Link:
  NP_002805
  Entrez Gene 53380 MouseEntrez Gene 116722 RatEntrez Gene 5716 Human
  UniProt ID O75832
• Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.
• Synonyms: dJ889N15.2; p28; p28(GANK)