TTLL12 Mouse Monoclonal Antibody [Clone ID: OTI13H9]

CAT#: TA500775S

Anti-TTLL12 mouse monoclonal antibody, clone OTI13H9 (formerly 13H9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTLL12 (RC200841, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12. Positive lysates LY402417 (100ug) and LC402417 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TTLL12 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-TTLL12 mouse monoclonal antibody (TA500775) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TTLL12 (RC200841).

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  HEK293T cells transfected with either pCMV6-ENTRY TTLL12 (RC200841) (Red) or empty vector control plasmid (Blue) were immunostained with anti-TTLL12 mouse monoclonal (TA500775, Dilution 1:1,000), and then analyzed by flow cytometry.

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  Flow cytometric analysis of Hela cells, using anti-TTLL12 antibody (TA500775), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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  Flow cytometric analysis of Jurkat cells, using anti-TTLL12 antibody (TA500775), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).

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Specifications

Product Data

• Clone Name: OTI13H9
• Applications: FC, IF, WB
• Recommended Dilution: IHC 1:100~200, WB: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TTLL12 (NP_055955) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.32 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.4 kDa
• Gene Name: tubulin tyrosine ligase like 12
• Database Link:
  NP_055955
  Entrez Gene 300105 RatEntrez Gene 23170 Human
  UniProt ID Q14166
• Synonyms: dJ526I14.2