IL6 Mouse Monoclonal Antibody [Clone ID: OTI2C9]

CAT#: TA500065

IL6 mouse monoclonal antibody, clone OTI2C9 (formerly 2C9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 (RC202078, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6. Positive lysates LY424617 (100ug) and LC424617 (20ug) can be purchased separately from OriGene.

• 

  IL-6 antibody (2C9) at 1:50000 + recombinant human IL-6 (Cat# RC202078) IL-6 antibody (2G3) at 1:2000

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500065)

Specifications

Product Data

• Clone Name: OTI2C9
• Applications: WB
• Recommended Dilution: WB 1:50000; IHC 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 29-212 of human IL-6 (NP_000591) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.5 kDa
• Gene Name: interleukin 6
• Database Link:
  NP_000591
  Entrez Gene 3569 Human
  UniProt ID P05231
• Background: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
• Synonyms: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Jak-STAT signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prion diseases, Toll-like receptor signaling pathway