SP1 Rabbit Monoclonal Antibody [Clone ID: R06-9I9]

CAT#: TA385307

SP1 Rabbit monoclonal Antibody

Product Images

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using SP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

• 

  Immunocytochemistry analysis of SP1(green) in Hela using SP1 antibody,and DAPI(blue)

Specifications

Product Data

• Clone Name: R06-9I9
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/100
  ICC/IF: 1/20
  IP: 1/20
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human SP1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 81 kDa; Observed MW: 90 kDa
• Gene Name: Sp1 transcription factor
• Database Link:
  Entrez Gene 6667 Human
  UniProt ID P08047
• Background: Swiss-Prot Acc.P08047.Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1.
• Synonyms: TSFP1