Foxo3 Rabbit Polyclonal Antibody

CAT#: TA376339

FOXO3A Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using FOXO3A antibody (TA376339) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 5s.

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  Immunofluorescence analysis of C6 cells using FOXO3A Rabbit pAb (TA376339) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH-3T3 cells using FOXO3A Rabbit pAb (TA376339) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 394-672 of mouse FOXO3A (NP_062714.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 71kDa
• Gene Name: forkhead box O3
• Database Link:
  Entrez Gene 56484 Mouse
  UniProt ID Q9WVH4
• Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
• Synonyms: AF6q21; DKFZp781A0677; FKHRL1; FKHRL1P2; FOXO2; FOXO3A; MGC12739; MGC31925