alpha Tubulin (TUBA1A) Rabbit Polyclonal Antibody

CAT#: TA355039

Rabbit Polyclonal Anti-Alpha-tubulin Antibody (biotin)

Product Images

Western blot analysis of Alpha-tubulin in multiple cell and tissue lysates with Biotin-Alpha-tubulin antibody at 1ug/ml. Lanes 1-20: 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, THP-1, U937, human brain, mouse brain, rat brain, rabbit brain, mouse lung, chicken small intestine, and zebrafish lysate, respectively.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1-2µg/ml.
• Reactivities: Human, Mouse, Rat, Rabbit, Chicken, Zebrafish
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Biotin-Alpha-tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-tubulin
• Formulation: Biotin-Alpha-tubulin antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Biotin-Alpha-tubulin antibody is affinity chromatography purified via peptide column.
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 50 kDa; Observed: 55 kDa
• Gene Name: tubulin alpha 1a
• Database Link:
  NP_006000
  Entrez Gene 22142 MouseEntrez Gene 64158 RatEntrez Gene 7846 Human
  UniProt ID Q71U36
• Background: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
• Synonyms: B-ALPHA-1; FLJ25113; hum-a-tub1; hum-a-tub2; LIS3; TUBA3
• Note: Biotin-Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 1 - 2 µg/ml.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection