gfp Rabbit Polyclonal Antibody

CAT#: TA354823

Rabbit anti GFP Polyclonal Antibody

Product Images

WB: The cell lysate of T293 with over-expressed recombinant protein GFP was resolved onto 10% SDSPAGE, then transferred onto NC membrane. Followed by an immune-blotting with Rabbit anti-GFP at 1:1000.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A full length GFP recombinant protein.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.9 kDa
• Background: The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.