PTEN Rabbit Polyclonal Antibody

CAT#: TA354666

Rabbit anti pTEN (Paired S380) Polyclonal Antibody

Product Images

WB: The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with Rabbit anti-PTEN (Paired S385) antibody (Lane 1) at 1:500, or with a pre-incubation of immunizing peptide (lane 2)

• 

  DB: 1 ug peptide was blot onto NC membrane A: PTEN (pS385) (Phosphospecific) B: PTEN (Paired S385) (Non phosphospeicfic) C: Non-related phosphospecific peptide were blotted at a 1:2000 dilution by: 1: Rabbit anti-PTEN (pS385) (Phosphospecific), 2: Rabbit anti-PTEN (Paired) (NonPhospho specific).

Specifications

Product Data

• Applications: Dot, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide surrounding to the epitope -DTSDP—without a phosphorylation site at Ser385 of human PTEN protein. This sequence is identical among human, mouse, rat , bovine and chicken origins.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58 kDa
• Gene Name: phosphatase and tensin homolog
• Database Link:
  NP_000305
  Entrez Gene 19211 MouseEntrez Gene 50557 RatEntrez Gene 5728 Human
  UniProt ID P60484
• Background: The phosphatase and tensin homologue (PTEN) tumor suppressor is a phosphatidylinositol D3-phosphatase that is frequently mutated in tumor cells. PTEN is itself regulated by phosphorylation on multiple serine and threonine residues in its C terminus. Casein kinase 2 (CK2) and Glycogen synthase kinase 3beta (GSK3beta), ATK/PI3K are involved in PTEN phosphorylation at sites of S370, S380, T382, T383, S385. The PTEN phosphorylation may play critical roles in the tumor suppressor functions.
• Synonyms: 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Endometrial cancer, Focal adhesion, Glioma, Inositol phosphate metabolism, Melanoma, p53 signaling pathway, Pathways in cancer, Phosphatidylinositol signaling system, Prostate cancer, Small cell lung cancer, Tight junction