beta Catenin (CTNNB1) Rabbit Polyclonal Antibody

SKU TA354649

Rabbit anti Catenin-beta (pS33) Polyclonal Antibody

Product Data

• Application: WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the epitope SGIHS with a single phosphorylation site Ser33 of human b-catenin.
• Buffer: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 88 kDa
• Gene Name: catenin beta 1
• Database Link:
  NP_001091679
  P35222
• Background: Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the β-catenin family. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin(s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, β-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3β and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates β-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of β-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3β activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3β, allowing unphosphorylated β-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.
• Synonyms: armadillo; CTNNB; MRD19

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway