Oct4 (POU5F1) Rabbit Polyclonal Antibody

CAT#: TA354593

Rabbit anti OCT-4 Polyclonal Antibody

Product Images

WB: The tissue lysate derived from mouse kidney was immunoprobed by Rabbit anti-OCT-4 at 1:500. An immunoreactive band is observed around ~34 kDa (1). This band is abolished by preincubation with immunizing peptide (2).

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  DB: 1 ug peptides was blotted onto NC membrane A: OCT-1 B: OCT-2 C: OCT-4 Followed an immunoblotting at a 1:1000 dilution by: 1: Rabbit anti-OCT-1 2: Rabbit anti-OCT-2 3: Rabbit anti-OCT-4 (601-450)

Specifications

Product Data

• Applications: Dot, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to intra domain of OCT-4 protein from human, mouse and rat origins.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39 kDa
• Gene Name: POU class 5 homeobox 1
• Database Link:
  NP_001167002
  Entrez Gene 18999 MouseEntrez Gene 294562 RatEntrez Gene 5460 Human
  UniProt ID Q01860
• Background: Oct-3/4, a 39 kDa protein as a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an Octamer motif ATGCAAAT. It is expressed in embryonic stem (ES) cells and germ cells, and its expression is required to sustain cell self-renewal and pluripotency. Oct-3/4 is the most recognized marker for totipotent ES cells.
• Synonyms: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors