Osteopontin (SPP1) Rabbit Polyclonal Antibody

CAT#: TA354515

Rabbit anti Osteopontin(SPP1) Polyclonal Antibody

Product Images

WB: The Cell lysate derived from HT-29 (Lane 2) were resolved onto 10% SDSPAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti-Osteopontin at 1:500. An immunoreactive band around ~34 kDa was observed.

• 

  IHC: Human breast cancer tissue stained with AntiRabbit anti-Osteopontin antibody at 1:100 for 10min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20min.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Rat, Mouse, Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the internal portion of Osteopontin protein sequence. This sequence is identical to human, rat and mouse origins.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~34 kDa
• Gene Name: secreted phosphoprotein 1
• Database Link:
  NP_000573
  Entrez Gene 20750 MouseEntrez Gene 25353 RatEntrez Gene 6696 Human
  UniProt ID P10451
• Background: Osteopontin (OPN or Early T-Lymphocyte Activation-1: Eta-1), also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, and minopotin, is a highly phosphorylated and glycosylated phosphoprtein that is expressed in many tissues. It is acidic, calcium-binding, cell surface-binding sequences (RGD)-containing protein was originally isolated from bone matrix. Osteopontin has been found in kidney, placenta, blood vessels. It is over-expressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.
• Synonyms: BNSP; BSPI; ETA-1; OPN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway