PKC alpha (PRKCA) Rabbit Polyclonal Antibody

CAT#: TA350903

Rabbit Polyclonal Anti-PRKCA Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-2: Jurkat cells NIH/3T3 cells Primary antibody: TA350903 (PRKCA Antibody) at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

• 

  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA350903 (PRKCA Antibody) at dilution 1/30 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA350903 (PRKCA Antibody) at dilution 1/30, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Jurkat and NIH/3T3 cells
  IHC: 25-100
  Positive control: Human lung cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human PRKCA
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77 kDa
• Gene Name: protein kinase C alpha
• Database Link:
  NP_002728
  Entrez Gene 18750 MouseEntrez Gene 24680 RatEntrez Gene 5578 Human
  UniProt ID P17252
• Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
• Synonyms: AAG6; PKC-alpha; PKCA; PRKACA

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Leukocyte transendothelial migration, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pathogenic Escherichia coli infection, Pathways in cancer, Phosphatidylinositol signaling system, Tight junction, Vascular smooth muscle contraction, VEGF signaling pathway, Vibrio cholerae infection, Wnt signaling pathway