HMG1 (HMGB1) Rabbit Polyclonal Antibody

CAT#: TA350691

Rabbit Polyclonal Anti-HMGB1 Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-5: Human hepatocellular carcinoma tissue Hela cells Jurkat cells 293T cells human breast infiltrative duct tissue Primary antibody: TA350691 (HMGB1 Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

• 

  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA350691 (HMGB1 Antibody) at dilution 1/30 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA350691 (HMGB1 Antibody) at dilution 1/30, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Human hepatocellsular carcinoma tissue, Hela and Jurkat cells, 293T cells and human breast infiltrative duct tissue
  IHC: 50-200
  Positive control: Human thyroid cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human HMGB1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25 kDa
• Gene Name: high mobility group box 1
• Database Link:
  NP_002119
  Entrez Gene 15289 MouseEntrez Gene 25459 RatEntrez Gene 3146 Human
  UniProt ID P09429
• Background: High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NF˚B family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.
• Synonyms: HMG1; HMG3; SBP-1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair