E2F1 Rabbit Polyclonal Antibody

CAT#: TA347842

Rabbit Polyclonal Anti-E2F-1 Antibody

Product Images

Western blot analysis of extracts from 3T3 cells using E2F-1 Antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-1:2000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-E2F-1 Antibody: Peptide sequence around aa.430~434(G-D-L-T-P) derived from Human E2F-1
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 ?.Stable for 12 months from date of receipt
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70kd
• Gene Name: E2F transcription factor 1
• Database Link:
  NP_005216
  Entrez Gene 13555 MouseEntrez Gene 1869 Human
  UniProt ID Q01094
• Background: Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. "P53 and E2F-1 cooperate to mediate apoptosis."
• Synonyms: E2F-1; RBAP1; RBBP3; RBP3
• Note: The antibody detects endogenous levels of total E2F-1 protein.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer