PADI4 Rabbit Polyclonal Antibody

CAT#: TA347254

Rabbit Polyclonal PADI4 Antibody

Product Images

WB was performed on nuclear extracts from HeLa cells (HeLa NE, 40 ug) with the antibody against PADI4, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk is shown on the left; the location of the protein of interest is indicated on the right.

• 

  Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody against PADI4. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40, 200.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:1,000-1:5,000); Western blotting (1:1,000)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-PADI4 antibody: human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: peptidyl arginine deiminase 4
• Database Link:
  NP_036519
  Entrez Gene 23569 Human
  UniProt ID Q9UM07
• Background: Protein arginine deiminases catalyse the conversion of arginine residues in proteins to citrulline. PADI4 (UniProt/Swiss-Prot entry Q9UM07) catalyses the citrullination-deimination of Arg-8 and Arg-17 from histone H3 and Arg-3 from histone H4. This prevents their methylation by CARM1 and HRMT1L2/PRMT1, thereby repressing transcription. PADI4 also citrullinates EP300/P300 at 'Arg-2142', which favours its interaction with NCOA2/GRIP.
• Synonyms: PAD; PAD4; PADI5; PDI4; PDI5