Bax inhibitor 1 (TMBIM6) Mouse Monoclonal Antibody [Clone ID: 20F565]

CAT#: TA337081

Mouse Monoclonal Bax inhibitor 1 Antibody (20F565)

Product Images

Western Blot: Bax inhibitor 1 Antibody (20F565) TA337081 - analysis of Bi-1 with this antibody. Lane 1: in vitro translated human Bi-1 protein. Lanes 2 and 3: IP/WB of Bi-1 protein enriched in the transgenic mouse brain (Lane 2) or wildtype brain (Lane 3). IP was done with a polyclonal Bi-1 antibody, followed by WB with this antibody.

• 

  Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) TA337081 - Formalin-fixed, paraffin-embedded human brain stained with Bi-1 antibody at 0.5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

• 

  Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) TA337081 - analysis of Bi-1 using Bi-1 antibody at 5 ug/ml: A) overview of stained human and mouse TMA, B-C) immunoreactivity in human archicortex (positive neurons of hippocampal gyrus) at 200X and 400X, D-E) strong immunoreactivity in neurons of human dorsal root ganglion.

• 

  Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) TA337081 - IHC analysis of formalin-fixed paraffin-embedded tissue section of human liver cancer using 5 ug/ml concentration of Bax inhibitor 1 antibody (clone 20F565). Cytoplasmic immuno-positivity of Bax inhibitor 1 (TMBIM6) was observed in the hepatis cancer cells whereas the cells of tumor stroma did not develop any staining [10X Magnification]

Specifications

Product Data

• Clone Name: 20F565
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry, Western Blot: 1-5 ug/ml, Immunohistochemistry-Paraffin: 0.5-5 ug/ml
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: A mixture of three synthetic peptides corresponding to the N-terminal, internal and C-terminal regions of human Bi-1. Epitope mapping indicates that the antibody recognizes an epitope in the N-terminal region of Bi-1
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transmembrane BAX inhibitor motif containing 6
• Database Link:
  NP_001092046
  Entrez Gene 24822 RatEntrez Gene 110213 MouseEntrez Gene 7009 Human
  UniProt ID P55061
• Synonyms: BAXI1; BI-1; TEGT

Reference Data

• Protein Families: Druggable Genome, Transmembrane