AMPK alpha 1 (PRKAA1) Mouse Monoclonal Antibody [Clone ID: 2B7]

SKU TA336924

Mouse Monoclonal AMPK alpha 1 Antibody (2B7)

Product Data

• Clone Name: 2B7
• Application: ELISA, FC, ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunohistochemistry-Paraffin: 1:200-1:1000, Simple Western: 1:50, Western Blot: 1:500, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Immunohistochemistry: 1:200 - 1:1000
• Reactivity: Human, Mouse, Primate, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131]
• Buffer: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 64 kDa
• Gene Name: protein kinase AMP-activated catalytic subunit alpha 1
• Database Link:
  NP_996790
  Q13131
• Background: The protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Synonyms: AMPK; AMPKa1
• Note: This AMPK alpha 1 (2B7) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy