| Application |
ChIP, ELISA, ICC/IF, IHC, IP, Simple Western, WB |
| Recommended Dilution |
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:50, Immunoprecipitation: 1:1000, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:500, ELISA: 1:1000 |
| Reactivity |
Human, Mouse |
| Antibody Host |
Rabbit |
| Clonality |
Polyclonal |
| Immunogen |
A synthetic peptide made to the human c-Myc protein (between residues 400-450) UniProt P01106 |
| Buffer |
PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Concentration |
lot specific |
| Purification |
Immunogen affinity purified |
| Conjugation |
Unconjugated |
| Storage |
Store at -20°C as received. |
| Stability |
Stable for 12 months from date of receipt. |
| Shipping |
Blue Ice |
| Gene Name |
v-myc avian myelocytomatosis viral oncogene homolog |
| Database Link |
|
| Background |
Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CACGATG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also. |
| Synonyms |
bHLHe39; c-Myc; MRTL; MYCC |
| Note |
This c-Myc antibody is useful for ELISA, Western Blot, Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation, and Immunohistochemistry-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. |
|
Reference Data
|
| Protein Categories |
Cancer: Brain and CNS, Cancer: Gastrointestinal, Cancer: Gynecologic, Cancer: Haematopoietic and Lymphoid, Cancer: Head and Neck, Cancer: Lung, Cancer: Sarcoma, Cancer: Skin, Growth Factors, Intracellular Proteins, Transciption Factors |
| Protein Families |
Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors |
| Protein Pathways |
Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway |
