SIAH1 Mouse Monoclonal Antibody [Clone ID: 8G7H12]

SKU TA336573

Mouse Monoclonal SIAH1/2 Antibody (8G7H12)

Product Data

• Clone Name: 8G7H12
• Application: IP, WB
• Recommended Dilution: Western Blot: 1:500, Immunoprecipitation: 1:10-1:500
• Reactivity: Drosophila, Human, Mouse, Porcine, Rat, Zebrafish
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide made to a C-terminal portion of the drosophila SINA protein (within residues 280-331). [Swiss-Prot# P21461]
• Buffer: Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
• Concentration: lot specific
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 37 kDa
• Gene Name: siah E3 ubiquitin protein ligase 1
• Database Link:
  NP_003022
  Q8IUQ4
• Background: The Drosophila SINA (seven in absentia) protein is an E3 ubiquitin ligase component of the RAS signal transduction pathway. The RAS signal pathway controls cell proliferation, differentiation, and survival, and regulation of this pathway is critical for normal development. In Drosophila SINA serves as a downstream gatekeeper required for proper RAS signal transduction. Similarly to SINA in Drosophila, the human protein SIAH has also been shown to be required for oncogenic RAS signaling in cancer.
• Synonyms: SIAH1A
• Note: This SIAH1/2 antibody is useful for Immunoprecipitation and Western blot, where a band is seen at ~37 kDa.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: p53 signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway