GAPDH Rabbit Polyclonal Antibody

CAT#: TA336478

Rabbit Polyclonal GAPDH/G3PDH Antibody

Product Images

Simple Western: GAPDH Antibody TA336478 - Lane view shows a specific band for GAPDH in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing A246conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight.

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  Western Blot: GAPDH Antibody TA336478 - Analysis using HeLa cells. Theoretical molecular weight: 36 kDa.

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  Western Blot: GAPDH Antibody TA336478 - Analysis of GAPDH in the multiple human tumor cell line lysate INSTA-Blot using this antibody. 25 ug/ml. Theoretical molecular weight: 36 kDa.

Specifications

Product Data

• Applications: ICC/IF, IHC, Immunoblotting, Simple Western, WB
• Recommended Dilution: Simple Western: 1:500, Immunohistochemistry, Western Blot: 1:500-1:2000, Immunoblotting, Immunocytochemistry/ Immunofluorescence
• Reactivities: Human, Mouse, Rat, Drosophila, Feline, Porcine, Protozoa
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Amino acids 73-87 PITIFQERDPSKIKW of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: glyceraldehyde-3-phosphate dehydrogenase
• Database Link:
  NP_002037
  Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 2597 Human
  UniProt ID P04406
• Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
• Synonyms: G3PD; GAPD; HEL-S-162eP

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways