IKB alpha (NFKBIA) Mouse Monoclonal Antibody [Clone ID: 39A1413]

CAT#: TA336464

Mouse Monoclonal IkB-alpha [p Ser32, p Ser36] Antibody (39A1413)

Product Images

Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) TA336464 - Analysis using the Biotin conjugate of TA336464. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with this antibody.

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  Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) TA336464 - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lane 2) or without 1 nM TNF-a (lane 1). The membrane was blotted with TA336464 and immunoreactivity was detected by ECL. The data shows that TA336464 detects specifically the phosphorylated form of IkBa.

Specifications

Product Data

• Clone Name: 39A1413
• Applications: IP, Simple Western, WB
• Recommended Dilution: Immunoprecipitation: 1-2 ug/ml, Western Blot: 1-2 ug/ml, Simple Western: 1:50
• Reactivities: Human, Mouse, Rat, Bovine, Canine, Porcine
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: NFKB inhibitor alpha
• Database Link:
  NP_065390
  Entrez Gene 18035 MouseEntrez Gene 25493 RatEntrez Gene 4792 Human
  UniProt ID P25963
• Background: NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes (7,8). Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBa.
• Synonyms: IKBA; MAD-3; NFKBI

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway