H4C14 Rabbit Polyclonal Antibody

CAT#: TA327358

TriMethyl-Histone H4-K20 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4K20me3 antibody.

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  Immunofluorescence analysis of 293T cell using H4K20me3 antibody. Blue: DAPI for nuclear staining.

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  Dot-blot analysis of all sorts of methylation peptides using H4K20me3 antibody.

Specifications

Product Data

• Applications: Dot, ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:50 - 1:200
• Reactivities: Human, Mouse, Rat, Other (Wide Range)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11 kDa
• Gene Name: histone cluster 2, H4a
• Database Link:
  Entrez Gene 8370 Human
  UniProt ID P62805
• Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
• Synonyms: FO108; H4; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4F2; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; HIST2H4; OTTHUMP00000013906; OTTHUMP00000194863