Arp3 (ACTR3) Rabbit Polyclonal Antibody

CAT#: TA326908

ACTR3 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ACTR3 antibody.

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  Immunofluorescence analysis of HeLa cell using ACTR3 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:10 - 1:100
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human ACTR3
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47 kDa
• Gene Name: ARP3 actin-related protein 3 homolog (yeast)
• Database Link:
  NP_005712
  Entrez Gene 74117 MouseEntrez Gene 10096 Human
  UniProt ID P61158
• Background: Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin. The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation. Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription.
• Synonyms: ARP3

Reference Data

• Protein Families: Druggable Genome