JUND Goat Polyclonal Antibody

CAT#: TA326732

Goat Anti-JUND Antibody

Product Images

TA326732 (1ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:64000; WB: 1-3µg/ml
• Reactivities: Mouse (Expected from sequence similarity: Human, Rat, Dog)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence CQLLPQHQVPAY, from the C Terminus of the protein sequence according to NP_005345.3.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: JunD proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  NP_005345
  Entrez Gene 16478 MouseEntrez Gene 24518 RatEntrez Gene 609409 DogEntrez Gene 3727 Human
  UniProt ID P17535
• Synonyms: AP-1
• Note: Approx 40kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 35.2kDa according to NP_005345.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Short and Pfarr,J Biol Chem. 2002 Sep 6;277(36):32697-705. PMID: 12105216). Recommended concentration: 1-3µg/ml. This antibody is expected NOT to cross-react with Jun oncogen (NP_002219.1, GeneID 3725)

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway