Superoxide Dismutase 1 (SOD1) Rabbit Polyclonal Antibody [Clone ID: N/A]

CAT#: TA326384

Rabbit polyclonal SOD (Cu/Zn) Antibody

Product Images

Western blot analysis of Cu/Zn SOD in a human cell line mix, using a 1:1000 dilution of the antibody

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  ICC localization of human Cu/Zn SOD in the syncytiotrophoblast of human placenta. Courtesy of Joan Telfer, University of Glasgow, UK.

Specifications

Product Data

• Clone Name: N/A
• Applications: IF, WB
• Recommended Dilution: 0.2ug/ml was sufficient for detection of Cu/Zn SOD in 20ug of HeLa cell lysate
• Reactivities: Human, Rat, Mouse, Bovine, Monkey, Dog, Hamster, Rabbit, Pig, Sheep, Xenopus, Coral
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human Cu/Zn SOD
• Formulation: PBS pH7.0, 50% glycerol, 0.09% sodium azide
• Concentration: lot specific
• Purification: Affinity (antigen) Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: superoxide dismutase 1, soluble
• Database Link:
  NP_000445
  Entrez Gene 20655 MouseEntrez Gene 24786 RatEntrez Gene 403559 DogEntrez Gene 574096 MonkeyEntrez Gene 6647 Human
  UniProt ID P00441
• Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body . It works by catalyzing the dismutation of the superoxide radical O2¯to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase . In general, SODs play a major role in antioxidant defense mechanisms . There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge . The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space. SOD3 can also be distinguished by its heparin-binding capacity .
• Synonyms: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD
• Note: Detects a ~23kDa (human) and 19kDa (other species) proteins corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS PAGE immunoblots.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Huntington's disease, Prion diseases