SMAD2 Rabbit Polyclonal Antibody

CAT#: TA324932

Rabbit polyclonal SMAD2 Antibody

Product Images

Western blot analysis of SMAD2 Antibody (Cat.# TA324932) in NIH-3T3 cell line lysates (35ug/lane). SMAD2 (arrow) was detected using the purified Pab.

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  IF image of SY5Y cells stained with SMAD2 antibody. SY5Y cells were incubated with TA324932 SMAD2 primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the SMAD2 mainly to the nucleus.

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  SMAD2 Antibody (Cat. #TA324932) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: IF: 1:100, WB: 1:1000, FC: 1:10~50
• Reactivities: Human, Mouse (Predicted: Rat, Zebrafish, Bovine, Chicken, Drosophila, Pig)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-125 amino acids from human SMAD2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52306 Da
• Gene Name: SMAD family member 2
• Database Link:
  NP_001003652
  Entrez Gene 17126 MouseEntrez Gene 29357 RatEntrez Gene 4087 Human
  UniProt ID Q15796
• Synonyms: hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway