PGP9.5 (UCHL1) Rabbit Polyclonal Antibody

CAT#: TA324710

Rabbit polyclonal UCHL1 Antibody (C-term)

Product Images

UCHL1 Antibody (C-term) (Cat.# TA324710) western blot analysis in U266, NCI-H1299, mouse Neuro-2a cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).

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  Western blot analysis of lysates from SH-SY5Y cell line and mouse brain tissue (from left to right), using UCHL1 Antibody (C-term) (Cat. #TA324710).TA324710 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

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  UCHL1 Antibody (C-term) (Cat. #TA324710)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UCHL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  UCHL1 Antibody (C-term) (Cat. #TA324710)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UCHL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of U251 cell stained with UCHL1 Antibody (C-term) (Cat#TA324710/SA120806AG).U251 cells were incubated with UCHL1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). UCHL1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, IHC: 1:10~50
• Reactivities: Human, Rat (Predicted: Mouse, Bovine, Pig, Monkey)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-214 amino acids from the C-terminal region of human UCHL1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24824 Da
• Gene Name: ubiquitin C-terminal hydrolase L1
• Database Link:
  NP_004172
  Entrez Gene 22223 MouseEntrez Gene 29545 RatEntrez Gene 701579 MonkeyEntrez Gene 7345 Human
  UniProt ID P09936
• Synonyms: HEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Parkinson's disease