HDAC1 Rabbit Polyclonal Antibody

CAT#: TA324515

Rabbit polyclonal anti-HDAC1 antibody, Loading control

Product Images

HDAC1 Antibody (N-term) (Cat. #TA324515) western blot analysis in MCF-7 cell line and human placenta and normal uterus, rat spleen lysates (35ug/lane).This demonstrates the HDAC1 antibody detected the HDAC1 protein (arrow).

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  IF image of MCF-7 cell stained with HDAC1 Antibody (N-term) (Cat#TA324515).MCF-7 cells were incubated with HDAC1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). HDAC1 immunoreactivity is localized to Nucleus significantly.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:1000, IF: 1:10 - 50
• Reactivities: Human, Rat (Predicted: Mouse, Bovine, Chicken, Xenopus)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human HDAC1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55103 Da
• Gene Name: histone deacetylase 1
• Database Link:
  NP_004955
  Entrez Gene 297893 RatEntrez Gene 433759 MouseEntrez Gene 3065 Human
  UniProt ID Q13547
• Synonyms: GON-10; HD1; RPD3; RPD3L1

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Huntington's disease, Notch signaling pathway, Pathways in cancer