FOXA2 Rabbit Polyclonal Antibody

CAT#: TA324382

Rabbit polyclonal FOXA2 Antibody (Center)

Product Images

FOXA2 Antibody (Center) (Cat. #TA324382) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FOXA2 antibody detected the FOXA2 protein (arrow).

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  FOXA2 Antibody (Center) (Cat. #TA324382)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOXA2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of A549 cell stained with FOXA2 Antibody (Center) (Cat#TA324382).A549 cells were incubated with FOXA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). FOXA2 immunoreactivity is localized to Nucleus significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, IF: 1:10~50
• Reactivities: Human (Predicted: Mouse, Rat)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This FOXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-157 amino acids from the Central region of human FOXA2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48306 Da
• Gene Name: forkhead box A2
• Database Link:
  NP_068556
  Entrez Gene 15376 MouseEntrez Gene 25099 RatEntrez Gene 3170 Human
  UniProt ID Q9Y261
• Synonyms: HNF3B; TCF3B

Reference Data

• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young