G protein alpha S (GNAS) Rabbit Polyclonal Antibody

CAT#: TA323636

Anti-GNAS Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Jurkat cells Primary antibody: TA323636 (GNAS Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 2 minutes

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA323636 (GNAS Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA323636 (GNAS Antibody) at dilution 1/20, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Jurkat cells
  IHC: 25-100
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to C terminal 200 amino acids of human GNAS complex locus
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: GNAS complex locus
• Database Link:
  NP_000507
  Entrez Gene 14683 MouseEntrez Gene 24896 RatEntrez Gene 2778 Human
  UniProt ID P84996
• Background: This locus has a highly complex imprinted expression pattern. It gives rise to maternally; paternally; and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons; and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus; and the antisense transcript; are paternally expressed noncoding RNAs; and may regulate imprinting in this region. In addition; one of the transcripts contains a second overlapping ORF; which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed; which results in different forms of the stimulatory G-protein alpha subunit; a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a; pseudohypoparathyroidism type 1b; Albright hereditary osteodystrophy; pseudopseudohypoparathyroidism; McCune-Albright syndrome; progressive osseus heteroplasia; polyostotic fibrous dysplasia of bone; and some pituitary tumors.?
• Synonyms: AHO; C20orf45; GNAS1; GPSA; GSA; GSP; NESP; PHP1A; PHP1B; PHP1C; POH; SgVI

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Calcium signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Long-term depression, Melanogenesis, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection