acyl CoA Thioesterase 2 (ACOT2) Rabbit Polyclonal Antibody

CAT#: TA322466

Anti-ACOT2 rabbit polyclonal antibody

Product Images

Predicted band size: 53 kDa. Positive control: MCF7 cell lysate. Recommended dilution: 1/100-500. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 minutes)

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  Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer using ACOT2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x 200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:500-2000, WB: 1:100-500,IHC: 1:15-50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 169-468 amino acids of human acyl-CoA thioesterase 2
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53 kDa
• Gene Name: acyl-CoA thioesterase 2
• Database Link:
  NP_006812
  Entrez Gene 171210 MouseEntrez Gene 192272 RatEntrez Gene 10965 Human
  UniProt ID P49753
• Background: This gene encodes a member of the acyl-CoA thioesterase protein family, and is one of four acyl-CoA hydrolase genes located in a cluster on chromosome 14. Alternative splicing results in multiple transcript variants. Acyl-CoA thioesterases, such as ACOT2, are a group of enzymes that hydrolyze Coenzyme A (CoA) esters, such as acyl-CoAs, bile CoAs, and CoA esters of prostaglandins, to the corresponding free acid and CoA. ACOT2 shows high acyl-CoA thioesterase activity on medium- and long-chain acyl-CoAs, with an optimal pH of 8.5. It is most active on myristoyl-CoA but also shows high activity on palmitoyl-CoA, stearoyl-CoA, and arachidoyl-CoA.
• Synonyms: CTE-IA; CTE1A; MTE1; PTE2; PTE2A; ZAP128

Reference Data

• Protein Pathways: Biosynthesis of unsaturated fatty acids