DNMT3A Rabbit Polyclonal Antibody

CAT#: TA322196

Anti-DNMT3A Rabbit Polyclonal Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-2: Hela and 231 cell lysates Primary antibody: TA322196 (DNMT3A Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 2 minutes

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA322196 (DNMT3A Antibody) at dilution 1/80 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA322196 (DNMT3A Antibody) at dilution 1/80, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA322196 (DNMT3A Antibody) at dilution 1/80 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA322196 (DNMT3A Antibody) at dilution 1/80, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Hela and 231 cell lysates
  IHC: 50-200
  Positive control: Human tonsil
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to C terminal 300 amino acids of human DNA (cytosine-5-)-methyltransferase 3 alpha
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78 kDa
• Gene Name: DNA (cytosine-5-)-methyltransferase 3 alpha
• Database Link:
  NP_072046
  Entrez Gene 13435 MouseEntrez Gene 444984 RatEntrez Gene 1788 Human
  UniProt ID Q9Y6K1
• Background: CpG methylation is an epigenetic modification that is important for embryonic development; imprinting; and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation; rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: DNMT3A2; M.HsaIIIA; TBRS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways