p27 KIP 1 (CDKN1B) Rabbit Polyclonal Antibody

CAT#: TA321617

Anti-CDKN1B Rabbit Polyclonal Antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-2: Jurkat and Raji cell lysates Primary antibody: TA321617 (CDKN1B Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 2 seconds

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  Immunohistochemistry of paraffin-embedded Human prostate cancer tissue using TA321617 (CDKN1B Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human prostate cancer tissue using TA321617 (CDKN1B Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Jurkat and Raji cell lysates
  IHC: 50-100
  Positive control: Human prostate cancer
  Predicted cell location: Cytoplasm and Nucleus
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length fusion protein
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22 kDa
• Gene Name: cyclin-dependent kinase inhibitor 1B
• Database Link:
  NP_004055
  Entrez Gene 12576 MouseEntrez Gene 1027 Human
  UniProt ID P46527
• Background: This gene encodes a cyclin-dependent kinase inhibitor; which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes; and thus controls the cell cycle progression at G1. The degradation of this protein; which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes; is required for the cellular transition from quiescence to the proliferative state.
• Synonyms: CDKN4; KIP1; MEN1B; MEN4; P27KIP1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer