Metabotropic Glutamate Receptor 8 (GRM8) Rabbit Polyclonal Antibody

CAT#: TA321210

Anti-GRM8 Rabbit Polyclonal Antibody

Product Images

Predicted band size: 56 kDa. Positive control: Mouse heart tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes)

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  Predicted cell location: Cytoplasm. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/150-500 The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using GRM8 antibody at dilution 1/150, on the right is treated with the synthetic peptide. (Original magnification:x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:2000-10000, WB: 1:1000-5000, IHC: 1:150-500
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 64-77 amino acids of Human glutamate receptor, metabotropic 8
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56 kDa
• Gene Name: glutamate metabotropic receptor 8
• Database Link:
  NP_000836
  Entrez Gene 14823 MouseEntrez Gene 60590 RatEntrez Gene 2918 Human
  UniProt ID O00222
• Background: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors; that have been divided into 3 groups on the basis of sequence homology; putative signal transduction mechanisms; and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4; GRM6; GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.?
• Synonyms: GLUR8; GPRC1H; mGlu8; MGLUR8

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction